Dr. Bowdish speaks about the role of the microbiome in inflammation and healthy/unhealthy aging (starting at 26:02). Other speakers include Dr. Luigi Ferrucci from the NIH Institute of Aging and Dr. James Kirkland who speaks about clinical trials testing senolytics.
This CIHR funded PDF position will study how aging, and specifically age-associated inflammation alters myeloid cell development and macrophage function. This will include performing flow cytometry assays to quantitate development and maturation of myeloid cells in human blood and depending on the applicant’s interest and aptitude may include animal models. The successful applicant will be expected to develop a research project including all required experimental optimization, liaise with collaborators and research participants from multiple sights and write manuscripts to communicate research findings. The applicant must have a background in immunology and be a team player who is willing to mentor junior trainees and be an active participant in departmental seminars and events.
• This project requires significant skills in flow cytometry. Although most of the work will be done in human blood, expertise in mouse models would also be an asset. Please include details of your flow cytometry experience in your cover letter and be prepared to discuss the details of protocol development, trouble shooting and optimization if you are chosen for an interview.
• The applicant will be required to work closely with our research participants, including obtaining consent and filling out detailed health questionnaires. This will require the ability to describe the research in lay terms and to work with older adults who may have issues with hearing and site. The applicant will also be required to liaise with research co-ordinators from multiple sites to facilitate shipments and answer technical questions. Excellent English skills are essential.
• The applicant will be expected to present research findings to the lay public, research coordinators, nurses and PIs and will need experience speaking to broad audiences. The applicant will be responsible for publishing manuscripts. Please describe your oral and written communication skills as well as your publication history (published and in preparation) in your cover letter.
Must be willing to learn:
• This successful applicant must be willing to take a phlebotomy course and take blood from our research participants.
BSL2 level blood processing
• The applicant will be handling human blood, including blood that may be infected with viruses and consequently will need to be committed to following sterile and safe practices.
• All our human immunology work is performed in a GLP compliant laboratory and the successful applicant must be willing to work with all the required GLP procedures.
• The applicant will perform ELISAs including multiplex ELISAs (e.g. Luminex).
Statistics/R programming language/Data visualization
• Analysis of complex datasets (e.g. multilinear regression) and the R programming language is essential.
Additional skills which may be an asset:
Animal models (e.g. chimeric bone marrow transplants)
• There will be opportunities to test hypotheses and models using the Preclinical Studies in Aging Laboratory (PSAL: www.psal.ca), Canada’s only aging mouse colony. Specifically, there are opportunities to study how the aging microenvironment alters myeloid development by performing heterochronic bone marrow chimeras.
• Experience in immunosenescence (mice and humans) would be a strong asset.
• Opportunities exist to collaborate on projects on how the aging immune system alters the upper respiratory tract and gut microbiota. Experience with analysis of 16s rRNA sequencing, statistics and large dataset visualization would be an asset.
Please describe your experience with any of these techniques in your cover letter.
Why the Bowdish lab?
Our lab’s core values are Diversity, Ambition, Innovation and Collaboration. These core values dictate our approach to doing science. We support our trainees career development for careers both inside and outside of academia and this project will provide skills that will be broadly desirable no matter what the career trajectory. The Bowdish lab supports scientists with families and diverse backgrounds (see our lab’s diversity statement at http://www.bowdish.ca/lab/lab-philosophy).
If you are interested in applying contact Dr. Bowdish at firstname.lastname@example.org. Please include a coverletter detailing your research interests ambitions and relevant skills and a c.v. that includes your publications and references.
Dr Bowdish is the Canadian Lung Association’s spokesperson for World Pneumonia Day (November 12, 2018). Here she discusses the importance of being vaccinated for pneumonia….
She also speaks to Zoomer Magazine about pneumonia, vaccinations and the aging immune system here…
To get a sense of the other lung research going on in the Bowdish lab, see our Instagram page: house.macrophage
Myeloid-derived suppressor cells (MDSCs) are immature myeloid cells whose
immunosuppressive activities contribute to cancer and other diseases. MDSCs
appear to increase with age, and this presumably contributes to immunosuppression
and the increased incidence of certain diseases. Why MDSCs increase with
age is not entirely clear. Herein we present evidence that MDSC expansion is due
in part to age-related changes in hematopoiesis, including the acquisition of
mutations that favor myelopoiesis, which are compounded by changes in the
aging microenvironment that favor the production of MDSCs.
Congratulations to the newly minted Dr. Loukov on successfully defending her thesis entitled “Age-Associated Inflammation impairs Myeloid Development and Monocyte & Macrophage Function”!
The Bowdish lab was very proud to host Anika Gupta, a high school student, for her Bay Area Science and Engineering Fair (BASEF) project.
Her project was entitled “Quantifying Lung Macrophages to Understand Increased Susceptibility to Bacterial Pneumonia with Age.”
Anita won the Dr. Doyle Biology Award for the best Biology project, a Gold merit award as well as the Pinnacle Award for the Third Best in Fair and a sponsored Trip Award to compete in the Intel International Science and Engineering Fair in Pittsburgh, Pennsylvania in May!
Way to go Anika!
See her featured in the Hamilton news here.…