General Protocols:

How to Write your first Manuscript

Calcein staining of macrophages

Cytokine ELISA

Macrophage Isolation & Culture:

Harvesting murine bone marrow

Isolation & culture of murine bone marrow macrophages

Propagation & culture of THP-1 cells

Cell signalling:

Nf-kB luciferase assay

RNA collection and gene expression analysis:

RNA extraction – lungs

RNA extraction – nasopharynx