General Biochemistry

Methylene Green SDS Detection Assay

  •  Is there SDS contamination in your reagent? Quantitate the amount of SDS using this assay based off of the traditional Methylene Blue assay.


  • This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.


 Coommassie blue staining of protein gels

Phenol-sulfuric acid Mannose detection assay.

  • This assay is sensitive to mannose from 1 – 100 𝜇g but will also detect other carbohydrates. We use this assay to quantify of complex carbohydrates in the bacterial cell wall