Cytokine ELISA 

  • This is a general protocol for performing ELISAs.

How to titrate antibodies for DIY ELISAs

  • It’s often cheaper to buy the components of an ELISA (i.e. standard, capture & detecting antibodies) to make your own ELISA than to buy commercial ones. Here is an easy to follow protocol on how to systematically determine if you are using the right concentrations of antibodies.

Specific ELISAs

Human C-reactive protein (CRP)