RNA extraction using TRIzol 

This protocol is used to extract the RNA from tissue or a cell sample using TRIzol and also includes recommendations for 260/280 and 260/230 values when measuring the concentration of extracted RNA using a nanodrop spectrometer.

RNA extraction – lungs

RNA extraction – nasopharynx

RNA Extraction For RNAseq

  • This protocol is for isolating RNA from cell lines or tissues for RNA sequencing.

RNA preparation for RNAseq (RNA sequencing/transcriptomics)

  • The protocol details the steps required fro processing (eukaryote) RNA for RNA sequencing experiments including ribosomal RNA depletion, quality control and quantification, cDNA preparation and labelling with adaptors. 

Amplification of 16s rRNA for Illumina sequencing