Growth and preparation of Streptococcus pneumoniae
Making sheep’s blood agar plates for culture of Streptococcus pneumoniae
Intranasal colonization of Streptococcus pneumoniae (and measurement of gene expression and leukocyte recruitment to the nasopharnyx)
CFSE labelling of S pneumoniae
- This protocol uses CFSE to label S. pneumoniae for flow cytometry or microscopy experiments.
Pneumococcal Cell Wall Purification
- This protocol describes how to purify the pneumococcal cell wall. The purified preparation will not contain proteins but will contain the peptidoglycan layer and teichoic acids.
Phenol-sulfuric acid Mannose detection assay.
- This assay is sensitive to mannose from 1 – 100 𝜇g but will also detect other carbohydrates. We use this assay to quantify of complex carbohydrates in the bacterial cell wall