Streptococcus pneumoniae

Growth and preparation of Streptococcus pneumoniae

Making sheep’s blood agar plates for culture of Streptococcus pneumoniae

Intranasal colonization of Streptococcus pneumoniae

 Nasopharyngeal colonization, and measurement of gene expression and leukocyte recruitment in the nasopharynx.
For a detailed protocol, see our paper here…

Puchta A, Verschoor CP, Thurn T, Bowdish DM. Characterization of inflammatory responses during intranasal colonization with Streptococcus pneumoniae. J Vis Exp. 2014 Jan 17;(83):e50490. doi: 10.3791/50490.

CFSE labelling of S pneumoniae

  • This protocol uses CFSE to label S. pneumoniae for flow cytometry or microscopy experiments.

Pneumococcal Cell Wall Purification

  • This protocol describes how to purify the pneumococcal cell wall. The purified preparation will not contain proteins but will contain the peptidoglycan layer and teichoic acids.

Phenol-sulfuric acid Mannose detection assay.

  • This assay is sensitive to mannose from 1 – 100 𝜇g but will also detect other carbohydrates. We use this assay to quantify of complex carbohydrates in the bacterial cell wall