Culturing LCM (L929 Culture media) for mouse macrophage culture
- L929 cells produce MCSF and other growth factors. Supernatant from L929 cultures can be used to differentiate macrophages.
Calcein staining of macrophages
- Labelling cells with calcein AM allows for them to be quantitated using a fluorescent plate reader and is useful for various in vitro assays, including chemotaxis assays
Griess assay for nitrate determination
- This assay is used for measuring nitric oxide production from macrophages (often used in macrophage polarization studies). Note that this assay must be done in RPMI media, not DMEM.