General Macrophage Protocols

Calcein staining of macrophages

  • Labelling cells with calcein AM allows for them to be quantitated using a fluorescent plate reader and is useful for various in vitro assays, including chemotaxis assays

Growing L929 cells and collecting conditioned media for macrophage culture

Griess assay for nitrate determination

  • This assay is used for measuring nitric oxide production from macrophages (often used in macrophage polarization studies). Note that this assay must be done in RPMI media, not DMEM.