An introduction to automated flow cytometry gating tools and their implementation
General Flow Cytometry protocol
Regulatory T cells (Tregs)
Myeloid Derived Suppressor Cells (both granulocytic and monocytic)
- Please note that MDSCs must be measured within 3 hours of taking blood.
Lymphocytes (T cells, B cells, NK cells)
T cells (naive, memory, TEMRA, senescent)
Sorting monocyte subsets for RNAseq
Flow cytometry protocol for staining Ly6Chigh (inflammatory)and Ly6Clow (patrolling) monocytes
Flow cytometry for bone marrow progenitors
- This stain can be used to identify hematopoetic stem cells, common lymphoid progenitors, common myeloid progenitors, megakaryocyte-erythroid progenitors and granulocyte/monocyte progenitors
Flow cytometry protocol for myeloid populations in the lung
T cells (naive, memory, TEMRA)
Bacterial flow cytometry
Measurement of binding to intact bacteria
- This protocol is used for “bacterial flow cytometry” (running bacteria through the flow cytometer rather than eukaroytic cells). We measure protein binding to the bacteria but it could easily be adapted to antibodies.