Harvesting murine bone marrow from femurs and tibia
- We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur & tibias.
- Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.
- Citation: Perry JA, et al. A macrophage-stimulating compound from a screen of microbial natural products.J Antibiot (Tokyo). 2015 Jan;68(1):40-6.
- The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture & propagation of the cells.
- Citation: Verschoor CP, et al. Anti-pneumococcal deficits of monocyte-derived macrophages from the advanced-age, frail elderly and related impairments in PI3K-AKT signaling.Hum Immunol. 2014 Dec;75(1\2):1192-6.