Murine macrophages

Harvesting murine bone marrow from femurs and tibia

Harvesting murine bone marrow from spines

• We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur & tibias. For an accompanying video, please contact Dr. Bowdish (bowdish@mcmaster.ca)

Culture, freezing and defrosting murine bone marrow macrophages

Elicitation and collection of peritoneal macrophages and neutrophils using Biogel

Cell lines

Propagation & culture of THP-1 cells

• Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.
• Citation: Perry JA, et al. A macrophage-stimulating compound from a screen of microbial natural products.J Antibiot (Tokyo). 2015 Jan;68(1):40-6.

 Culturing RAW264.7 Cells

• The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture & propagation of the cells.

Human

Human monocyte derived macrophage culture

• Citation: Verschoor CP, et al. Anti-pneumococcal deficits of monocyte-derived macrophages from the advanced-age, frail elderly and related impairments in PI3K-AKT signaling.Hum Immunol. 2014 Dec;75(1\2):1192-6.