Macrophage Isolation & Culture

Murine macrophages

Harvesting murine bone marrow from femurs and tibia

Harvesting bone marrow from spines

Harvesting murine bone marrow from spines

  • We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur & tibias. For an accompanying video, please contact Dr. Bowdish (bowdish@mcmaster.ca)

Culture, freezing and defrosting murine bone marrow macrophages

Elicitation and collection of peritoneal macrophages and neutrophils using Biogel

Cell lines

Propagation & culture of THP-1 cells

 Culturing RAW264.7 Cells

  • The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture & propagation of the cells.

Human

Human monocyte derived macrophage culture