Cell/Tissue Culture

 Counting cells with a hemocytometer and measuring viability with Trypan Blue. 

Propagation & culture of THP-1 cells

  •  Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.

 Culturing RAW 264.7 Cells

  • The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. This is an update of the original in we describe culture & propogation of the cells and include information on seeding density for studies of cytokine production.

 Culturing HEK293/HEK293T cells

Culturing, Maintaining and Seeding the nasopharyngeal cell line Detroit 562

  • This human derived nasopharyngeal carcinoma cell line maintains many of the properties of nasopharyngeal epithelial cells.

Growing L929 cells and collecting conditioned media for macrophage culture