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Bowdish Lab

Macrophage Biology at McMaster University

Bowdish Lab

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    • Uncovering how age-associated inflammation alters the maturation and function of monocytes and macrophages and how these changes increase susceptibility to pneumonia
    • Dissecting the complex interplay between host immune cells and the microbiota of the upper respiratory tract
    • Macrophage scavenger receptors in host defence
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Detection of Bacterial Products

Measuring LPS in plasma or serum

  • This protocol is used for measuring levels of LPS in mouse or human plasma or serum.
  • Citation: Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007/s10753-014-0030-z.

Measuring DNA in plasma or serum (16s rRNA, mitochondrial, nuclear)

  • Citation: Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007/s10753-014-0030-z.

Measuring muramyl dipeptide (MDP) in plasma or serum

  • This protocol is used for measuring levels of MDP, a component of the bacterial cell wall in mouse or human plasma or serum.
  • Citation: Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, …Cell Host & Microbe 21 (4), 455-466. e4

Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells

  • This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.

NF-KB Luciferase Assay using HEK293T cells

  • This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.

Dawn Bowdish

Recent Lab News

  • Dr. Bowdish speaks on TVO’s “The Agenda” What is the future of COVID-19 vaccines?
  • Bowdish lab is not accepting thesis students for 2022/2023
  • SARS-CoV-2 and back to school- 2021
  • Mactalks: Outbreaks, vaccines & immunity: Inside long-term care with Andre Picard & Andrew Costa
  • Dr. Bowdish talks about saliva testing for COVID-19

Recent Tweets

MsMacrophageDawn Bowdish@MsMacrophage·
10h

So happy to see my collaborator, @Monica97026710 , recognized with this honour - she’s brilliant!

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Retweet on TwitterDawn Bowdish Retweeted
CovidInltcCOVID in LTC Study@CovidInltc·
28 Jun

Thank you, George, for participating in our study! Because of participants like you we can do the important research needed to help residents get back to having safe visits with family and friends!

Let’s Fight to Stop COVID Together!!!

Reply on Twitter 1541838275165523968Retweet on Twitter 15418382751655239681Like on Twitter 15418382751655239686Twitter 1541838275165523968
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Micheal G. DeGroote Institute for Infectious Disease Research

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