Measuring LPS in plasma or serum

• This protocol is used for measuring levels of LPS in mouse or human plasma or serum.
• Citation: Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007/s10753-014-0030-z.

Measuring muramyl dipeptide (MDP) in plasma or serum

• This protocol is used for measuring levels of MDP, a component of the bacterial cell wall in mouse or human plasma or serum.
• Citation: Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, …Cell Host & Microbe 21 (4), 455-466. e4

Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells

• This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.

NF-KB Luciferase Assay using HEK293T cells

• This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.