General
This protocol describes the process by which bone marrow chimera mouse models are generated in the Bowdish research group from spinal bone marrow (BM) donors.
Using FITC-dextran to measure intestinal permeability
- This protocol uses FITC-dextran to measure intestinal permeability. If you use this protocol, please cite: Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, …Cell Host & Microbe 21 (4), 455-466. e4
Genotyping
- This protocol is for genotyping TNF KO derived from heterozygote breeding
Genotyping Scavenger Receptor A and MARCO (Msr1 & Marco) knockout mice
Behaviour and Cognition Tests
The novel object recognition test is used to evaluate recognition memory in mice. The test consists of two sessions in the open field arena, separated by an intersession interval (ISI). In the first session, the Training, the mouse is free to explore and familiarize itself with two identical objects; during the second session, Testing, one of the now-familiar objects is replaced by a novel object. A mouse that remembers the familiar object will spend more time exploring the novel one. By using a short ISI (3 hours), this test assesses short-term recognition memory.
The Open Field test measures anxiety, willingness to explore, and general mobility and behaviour.
This protocol describes the process by which bone marrow chimera mouse models are generated in the Bowdish research group from spinal bone marrow (BM) donors.
Measuring intestinal permeability
Using FITC-dextran to measure intestinal permeability
- This protocol uses FITC-dextran to measure intestinal permeability. If you use this protocol, please cite: Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, …Cell Host & Microbe 21 (4), 455-466. e4
Measuring frailty