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Bowdish Lab

Macrophage Biology at McMaster University

Bowdish Lab

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  • The Lab
  • Projects
    • Vaccination and infection in older adults
    • Age, pneumonia, and post-pneumonia health issues
    • The role of the microbiota in healthy/unhealthy aging
    • Uncovering how age-associated inflammation alters the maturation and function of monocytes and macrophages.
    • General Macrophage Publications
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Detection of Bacterial Products

Measuring LPS in plasma or serum

  • This protocol is used for measuring levels of LPS in mouse or human plasma or serum.
  • Citation: Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007/s10753-014-0030-z.

Measuring DNA in plasma or serum (16s rRNA, mitochondrial, nuclear)

  • Citation: Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007/s10753-014-0030-z.

Measuring muramyl dipeptide (MDP) in plasma or serum

  • This protocol is used for measuring levels of MDP, a component of the bacterial cell wall in mouse or human plasma or serum.
  • Citation: Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, …Cell Host & Microbe 21 (4), 455-466. e4

Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells

  • This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.

NF-KB Luciferase Assay using HEK293T cells

  • This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.

Dawn Bowdish

Recent Lab News

  • The Agenda: Why aren’t more kids vaccinated against measles? With Dr. Bowdish and other experts.
  • Publication: Minimal Impact of Prior Common Cold Coronavirus Exposure on Immune Responses to Severe Acute Respiratory Syndrome Coronavirus 2 Vaccination or Infection Risk in Older Adults in Congregate Care
  • Post-doctoral position in Human Immunology Available
  • The Bowdish lab is no longer accepting thesis student applications for the 2025/26 academic year.
  • Publication: Canadian Immunity Task Force (CITF) Hema-Net Serosurveillance Meeting
  • “The Perils of Being Born in the Fall” will be at the Zoetic Theatre November, 12, 2024
  • Dr. Bowdish speaks at the 2024 Innovation Nation Conference & Student Innovation Showcase
  • Dr. Bowdish discusses COVID-19 on The Agenda with Steve Paikin
  • Publication: Reassuring humoral and cellular immune responses to SARS-CoV-2 vaccination in participants with systemic sclerosis
  • Publication: “Monocyte-driven inflamm-aging reduces intestinal barrier function in females”

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