These are protocols that are used in the Bowdish lab. We hope that these are in sufficient detail as to be helpful for you but if not please feel free to contact Dr. Bowdish at bowdish@mcmaster.ca. We update these frequently in the hopes of providing the community with clear, concise and easy-to-reproduce protocols.

• Antibody production and purification
• Blood Processing & Leukocyte Isolation
• Bowdish Lab-Specific
• Cytokine measurements (ELISA & others)
  • General
  • Specific ELISAs
• Detection of Bacterial Products
• Flow Cytometry
  • General
  • Human
  • Murine
  • Bacteria
• General Biochemistry & Molecular Biology
  • Cloning & Transfection
  • PCR
  • Protein
  • RNA
  • Other (e.g., bacterial sugars, SDS contamination)
• General Lab protocols
• Macrophage isolation and culture (primary, for cell lines see ‘Tissue Culture’)
  • Murine
  • Human
• Metabolomics & Mass Spec
• Microbiology & Streptococcus pneumoniae protocols
• Phagocytosis & Endocytosis 
• PreClinical Studies on Aging Protocols
  • General
  • Genotyping
  • Behaviour and Cognition Tests
• Scavenger Receptors
• Tissue Collection & Processing
• Tissue Culture and Cell Lines
• Writing & Presenting