Macrophage Isolation & Culture:

Harvesting murine bone marrow

Isolating Bone Marrow from Femurs

Isolation & culture of murine bone marrow macrophages

Propagation & culture of THP-1 cells

•  Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.

Calcein staining of macrophages

Culturing RAW264.7 Cells

• The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture & propogation of the cells.