General

An introduction to automated flow cytometry gating tools and their implementation

• This review on automated gating tool for use in multi-parameter flow cytometry is published in Front. Immunol. 6:380. doi: 10.3389/fimmu.2015.00380

General Flow Cytometry protocol

Bacterial flow cytometry

Measurement of binding to intact bacteria

• This protocol is used for “bacterial flow cytometry” (running bacteria through the flow cytometer rather than eukaroytic cells). We measure protein binding to the bacteria but it could easily be adapted to antibodies.

Humans

 Human monocyte staining protocol

 Human lymphocyte staining protocol

Human Treg (regulatory T cell) staining

Mice

Flow cytometry protocol for staining Ly6Chigh and Ly6Clow monocytes