Preparation of eukaryote and prokaryote cells for analysis of the endo- or exo-metabolome by Liquid Chromatography Mass Spec

• The detailed protocol has been published here (click link for .pdf)….Comprehensive and simultaneous coverage of lipid and polar metabolites for endogenous cellular metabolomics using HILIC-TOF-MS. Fei F, Bowdish DM, McCarry BE. Anal Bioanal Chem. 2014 Jun;406(15):3723-33. doi: 10.1007/s00216-014-7797-5. PMID: 24714971
• This methodology was used to investigate macrophage metabolism here…Age-associated metabolic dysregulation in bone marrow-derived macrophages stimulated with lipopolysaccharide. Fei F, Lee KM, McCarry BE, Bowdish DM.Sci Rep. 2016 Mar 4;6:22637. doi: 10.1038/srep22637. PMID: 26940652
• This methodology was used to measure microbial metabolism here (slight differences in extraction methods)...Fei F, Mendonca ML, McCarry BE, Bowdish DM, Surette MG. Metabolic and transcriptomic profiling of Streptococcus intermedius during aerobic and anaerobic growth. Metabolomics. 2016 Mar 1;12(3):46.