Counting cells with a hemocytometer and measuring viability with Trypan Blue.
- L929 cells produce MCSF and other growth factors. Supernatant from L929 cultures can be used to differentiate macrophages.
THP-1 cells (human monocyte-like cells)
- Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.
- Citation: Perry JA, et al. A macrophage-stimulating compound from a screen of microbial natural products.J Antibiot (Tokyo). 2015 Jan;68(1):40-6.
RAW264.7 Cells (mouse peritoneal-derived macrophage cell line)
- The murine RAW 264.7 cell line is considered one of the best macrophage cell lines.
Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells
- This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.
NF-KB Luciferase Assay using HEK293T cells
- This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.
Nasopharyngeal cell line Detroit 562
- This human derived nasopharyngeal carcinoma cell line maintains many of the properties of nasopharyngeal epithelial cells.