{"id":661,"date":"2012-08-16T13:32:07","date_gmt":"2012-08-16T17:32:07","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=661"},"modified":"2012-09-02T08:56:58","modified_gmt":"2012-09-02T12:56:58","slug":"macrophage-function","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-function\/","title":{"rendered":"Macrophage function"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/07\/Macrophage-Killing-Assay.pdf\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/>Macrophage Killing Assay<\/a><\/p>\n<ul>\n<li>How do you measure how well macrophages kill bacteria (or whether your treatment alters killing)? This is a standard kill curve protocol and a workhorse of the Bowdish lab. Specifically written to measure\u00a0<em>S. pneumoniae<\/em>\u00a0killing, can be adapted for any bacteria and any type of macrophage (e.g. human, murine, alveolar, bone marrow,etc)<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/07\/Macrophage-Killing-Assay.pdf\">.<\/a><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">Calcein staining of<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">\u00a0macrophages<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Macrophage Killing Assay How do you measure how well macrophages kill bacteria (or whether your treatment alters killing)? This is a standard kill curve protocol and a workhorse of the Bowdish lab. Specifically written to measure\u00a0S. pneumoniae\u00a0killing, can be adapted &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-function\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-661","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/661","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=661"}],"version-history":[{"count":8,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/661\/revisions"}],"predecessor-version":[{"id":664,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/661\/revisions\/664"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=661"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}