{"id":445,"date":"2011-08-14T22:51:04","date_gmt":"2011-08-15T02:51:04","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=445"},"modified":"2013-09-03T10:57:10","modified_gmt":"2013-09-03T14:57:10","slug":"rna-collection-expression","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/rna-collection-expression\/","title":{"rendered":"RNA Collection &#038; Expression"},"content":{"rendered":"<p><em>RNA collection and gene expression analysis:<\/em><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/RNA_extraction_lungs.pdf\">RNA extraction &#8211; lungs<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/RNA_extraction-nasopharnyx.pdf\">RNA extraction &#8211; nasopharynx<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/06\/RNA-Extraction-For-RNAseq.pdf\">RNA Extraction For RNAseq<\/a><\/p>\n<ul>\n<li>This protocol is for isolating RNA from cell lines or tissues for RNA sequencing.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/09\/Sample-Preparation-For-RNAseq.pdf\">RNA preparation for RNAseq<\/a>\u00a0(RNA sequencing\/transciptomics)<\/p>\n<ul>\n<li>The protocol details the steps required fro processing (eukaryote) RNA for RNA sequencing experiments including ribosomal RNA depletion, quality control and quantification, cDNA preparation and labelling with adaptors.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>RNA collection and gene expression analysis: RNA extraction &#8211; lungs RNA extraction &#8211; nasopharynx RNA Extraction For RNAseq This protocol is for isolating RNA from cell lines or tissues for RNA sequencing. RNA preparation for RNAseq\u00a0(RNA sequencing\/transciptomics) The protocol details &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/rna-collection-expression\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":632,"parent":436,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-445","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/445","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=445"}],"version-history":[{"count":7,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/445\/revisions"}],"predecessor-version":[{"id":1204,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/445\/revisions\/1204"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/632"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=445"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}