{"id":441,"date":"2011-08-14T22:46:46","date_gmt":"2011-08-15T02:46:46","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=441"},"modified":"2025-11-15T08:17:27","modified_gmt":"2025-11-15T13:17:27","slug":"macrophage-isolation-culture","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-isolation-culture\/","title":{"rendered":"Macrophage Isolation &#038; Culture"},"content":{"rendered":"<p><em>Macrophage Isolation &amp; Culture:<\/em><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Harvesting_murine_bone-marrow.pdf\">Harvesting murine bone marrow<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2025\/11\/protocol-femur-BM-extraction-2024-11-14.pdf\">Isolating Bone Marrow from Femurs<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Isolation_and_culture_of_murine_bone_marrow_macrophages.pdf\">Isolation &amp; culture of murine bone marrow macrophages<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/THP-1-propagation-culture.pdf\">Propagation &amp; culture of THP-1 cells<\/a><\/p>\n<ul>\n<li>\u00a0Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">Calcein staining of<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">\u00a0macrophages<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/02\/Culturing-RAW264.7-Cells.pdf\">Culturing RAW264.7 Cells<\/a><\/p>\n<ul>\n<li>The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture &amp; propogation of the cells.<\/li>\n<\/ul>\n<p><em>\u00a0<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Macrophage Isolation &amp; Culture: Harvesting murine bone marrow Isolating Bone Marrow from Femurs Isolation &amp; culture of murine bone marrow macrophages Propagation &amp; culture of THP-1 cells \u00a0Tips and tricks for growing this popular, but finicky, human monocyte-like cell line. &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-isolation-culture\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":3,"featured_media":632,"parent":436,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-441","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/441","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=441"}],"version-history":[{"count":9,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/441\/revisions"}],"predecessor-version":[{"id":3728,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/441\/revisions\/3728"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/632"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=441"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}