{"id":2742,"date":"2018-12-30T22:17:21","date_gmt":"2018-12-31T03:17:21","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2742"},"modified":"2026-01-03T13:59:50","modified_gmt":"2026-01-03T18:59:50","slug":"cell-tissue-culture","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/cell-tissue-culture\/","title":{"rendered":"Cell\/Tissue Culture"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Cell-Count.pdf\">Counting cells with a hemocytometer and measuring viability with Trypan Blue.\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2021\/09\/protocol-lcm-harvest-2021-09-23.pdf\">LCM (L929 Culture media) <\/a><\/p>\n<ul>\n<li>L929 cells produce MCSF and other growth factors. Supernatant from L929 cultures can be used to differentiate macrophages.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/THP-1-propagation-culture.pdf\">THP-1 cells<\/a> (human monocyte-like cells)<\/p>\n<ul>\n<li>Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.<\/li>\n<li>Citation:\u00a0<a href=\"https:\/\/www.nature.com\/articles\/ja201483\">Perry JA, et al. A macrophage-stimulating compound from a screen of microbial natural products.J Antibiot (Tokyo). 2015 Jan;68(1):40-6<\/a>.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/02\/Culturing-RAW264.7-Cells.pdf\">RAW264.7 Cells<\/a> (mouse peritoneal-derived macrophage cell line)<\/p>\n<ul>\n<li>The murine RAW 264.7 cell line is considered one of the best macrophage cell lines.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/SEAP-Reporter-Assay.pdf\">Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells<\/a><\/p>\n<ul>\n<li>This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/07\/NF-KB-Luciferase-Assay-Protocolv2.pdf\">NF-KB Luciferase Assay using HEK293T cells<\/a><\/p>\n<ul>\n<li>This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/04\/Culturing-Maintaining-and-Seeding-Detroit-562-Cells-1.pdf\">Nasopharyngeal cell line Detroit 562<\/a><\/p>\n<ul>\n<li>This\u00a0human derived nasopharyngeal carcinoma cell line maintains many of the properties of nasopharyngeal epithelial cells.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Counting cells with a hemocytometer and measuring viability with Trypan Blue.\u00a0 LCM (L929 Culture media) L929 cells produce MCSF and other growth factors. Supernatant from L929 cultures can be used to differentiate macrophages. THP-1 cells (human monocyte-like cells) Tips and &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/cell-tissue-culture\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2742","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2742","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2742"}],"version-history":[{"count":3,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2742\/revisions"}],"predecessor-version":[{"id":3769,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2742\/revisions\/3769"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2742"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}