{"id":2736,"date":"2018-12-30T22:15:37","date_gmt":"2018-12-31T03:15:37","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2736"},"modified":"2026-01-03T13:46:21","modified_gmt":"2026-01-03T18:46:21","slug":"metabolomics-mass-spec","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/metabolomics-mass-spec\/","title":{"rendered":"Metabolomics &#038; Mass Spec"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/06\/Cell-Preparation-for-LCMS.pdf\">Preparation of eukaryote and prokaryote cells for analysis of the endo- or exo-metabolome by Liquid Chromatography Mass Spec<\/a><\/p>\n<ul>\n<li>The detailed protocol has been published here (click link for .pdf)&#8230;<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/2016-Fei-Metabolomics-2016-122-1-14-3.pdf\">.Comprehensive and simultaneous coverage of lipid and polar metabolites for endogenous cellular metabolomics using HILIC-TOF-MS<\/a>. Fei F, Bowdish DM, McCarry BE. Anal Bioanal Chem. 2014 Jun;406(15):3723-33. doi: 10.1007\/s00216-014-7797-5. PMID: 24714971<\/li>\n<li>This methodology was used to investigate macrophage metabolism here&#8230;<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/Fei-Macrophage-metabolism-srep22637.pdf\">Age-associated metabolic dysregulation in bone marrow-derived macrophages stimulated with lipopolysaccharide.<\/a> Fei F, Lee KM, McCarry BE, Bowdish DM.Sci Rep. 2016 Mar 4;6:22637. doi: 10.1038\/srep22637. PMID: 26940652<\/li>\n<li>This methodology was used to measure microbial metabolism here (slight differences in extraction methods)..<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/Fei-et-al-SMG-Metabolomics.pdf\">.Fei F, Mendonca ML, McCarry BE, Bowdish DM, Surette MG. Metabolic and transcriptomic profiling of Streptococcus intermedius during aerobic and anaerobic growth<\/a>. Metabolomics. 2016 Mar 1;12(3):46.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Preparation of eukaryote and prokaryote cells for analysis of the endo- or exo-metabolome by Liquid Chromatography Mass Spec The detailed protocol has been published here (click link for .pdf)&#8230;.Comprehensive and simultaneous coverage of lipid and polar metabolites for endogenous cellular &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/metabolomics-mass-spec\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2736","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2736","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2736"}],"version-history":[{"count":3,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2736\/revisions"}],"predecessor-version":[{"id":3761,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2736\/revisions\/3761"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2736"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}