{"id":2731,"date":"2018-12-30T22:01:56","date_gmt":"2018-12-31T03:01:56","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2731"},"modified":"2019-08-07T21:43:18","modified_gmt":"2019-08-08T01:43:18","slug":"protein-gels-western-blots","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/protein-gels-western-blots\/","title":{"rendered":"Protein Gels &#038; Western Blots"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Immunoprecipitation.pdf\">Immunoprecipitation<\/a><\/p>\n<ul>\n<li>This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Co-Immunoprecipitation.pdf\">Co-immunoprecipitation<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2019\/08\/Updated-Western-blot-protocol.pdf\">Western immunoblotting<\/a><\/p>\n<ul>\n<li>This includes running the gel, transferring to PVDF membrane and preparing antibodies.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Coomassie-Blue-Stain-Protocol.pdf\">Coommassie blue staining of protein gels<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Immunoprecipitation This protocol demonstrates how to immunoprecipitate a protein from a cell lysate. Co-immunoprecipitation Western immunoblotting This includes running the gel, transferring to PVDF membrane and preparing antibodies. \u00a0Coommassie blue staining of protein gels<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2731","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2731","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2731"}],"version-history":[{"count":4,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2731\/revisions"}],"predecessor-version":[{"id":2880,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2731\/revisions\/2880"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2731"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}