{"id":2729,"date":"2018-12-30T21:58:57","date_gmt":"2018-12-31T02:58:57","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2729"},"modified":"2018-12-30T21:58:57","modified_gmt":"2018-12-31T02:58:57","slug":"general-biochemistry","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/general-biochemistry\/","title":{"rendered":"General Biochemistry"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/08\/Methylene-Green-SDS-Detection-Assay-4.pdf\">Methylene Green SDS Detection Assay<\/a><\/p>\n<ul>\n<li>\u00a0Is there SDS contamination in your reagent? Quantitate the amount of SDS using this assay based off of the traditional Methylene Blue assay.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Immunoprecipitation.pdf\">Immunoprecipitation<\/a><\/p>\n<ul>\n<li>This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Co-Immunoprecipitation.pdf\">Co-immunoprecipitation<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Coomassie-Blue-Stain-Protocol.pdf\">Coommassie blue staining of protein gels<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/08\/Pneumoncoccal-Cell-Wall-Purification-2DB-edits.pdf\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Mannose-Detection-Protocol.pdf\">Phenol-sulfuric acid Mannose detection assay<\/a>.<\/p>\n<ul>\n<li>This assay is sensitive to mannose from 1 \u2013 100 ?g but will also detect\u00a0other carbohydrates. We use this assay to\u00a0quantify of complex carbohydrates in the bacterial cell wall<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Methylene Green SDS Detection Assay \u00a0Is there SDS contamination in your reagent? Quantitate the amount of SDS using this assay based off of the traditional Methylene Blue assay. Immunoprecipitation This protocol demonstrates how to immunoprecipitate a protein from a cell &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/general-biochemistry\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2729","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2729","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2729"}],"version-history":[{"count":1,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2729\/revisions"}],"predecessor-version":[{"id":2730,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2729\/revisions\/2730"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2729"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}