{"id":2722,"date":"2018-12-30T21:48:14","date_gmt":"2018-12-31T02:48:14","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2722"},"modified":"2023-04-24T16:09:08","modified_gmt":"2023-04-24T20:09:08","slug":"pcr","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/pcr\/","title":{"rendered":"PCR"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/04\/qPCR-Bible.pdf\">The PCR resource manual.<\/a><\/p>\n<ul>\n<li>The how-to guide:qPCR from primer design to testing those primers in preparation for quantitative PCR<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" style=\"color: #000000; font-weight: bold;\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2023\/04\/Updated-cDNA-Synthesis-Protocol-Aunika-Venables.pdf\">cDNA synthesis<\/a><\/p>\n<ul>\n<li>This protocol is used to synthesis cDNA from isolated RNA using the SuperScript II reverse transcriptase enzyme with the aim to use the synthesized cDNA in subsequent 2-stage RT-qPCR reactions.<\/li>\n<\/ul>\n<p><em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<\/em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/08\/qPCR-Primer-Design.pdf\">Designing primers for qPCR<\/a><\/p>\n<p><em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<\/em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2023\/04\/Updated-qPCR-Protocol-Aunika-Venables.pdf\">Two-step qPCR<\/a><\/p>\n<p>Two-step reverse transcription quantitative polymerase chain reaction, often abbreviated as 2-Step RT-qPCR or simply as qPCR, is a high-throughput assay design that allows the real-time measurement of the expression level of specific genes at the RNA level. This protocol was written for SYBR green.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The PCR resource manual. The how-to guide:qPCR from primer design to testing those primers in preparation for quantitative PCR cDNA synthesis This protocol is used to synthesis cDNA from isolated RNA using the SuperScript II reverse transcriptase enzyme with the &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/pcr\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2722","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2722","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2722"}],"version-history":[{"count":3,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2722\/revisions"}],"predecessor-version":[{"id":3300,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2722\/revisions\/3300"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2722"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}