{"id":2720,"date":"2018-12-30T21:45:53","date_gmt":"2018-12-31T02:45:53","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2720"},"modified":"2018-12-30T21:45:53","modified_gmt":"2018-12-31T02:45:53","slug":"general-molecular-biology","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/general-molecular-biology\/","title":{"rendered":"General Molecular Biology"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Molecular-Cloning.pdf\">Cloning<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/04\/Site-Directed-Mutagenesis-Protocol.pdf\">Site-directed mutagenesis<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Immunoprecipitation.pdf\">Immunoprecipitation<\/a><\/p>\n<ul>\n<li>This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Co-Immunoprecipitation.pdf\">Co-immunoprecipitation<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/01\/PEI_Transfection_Bowdish_v2.pdf\">Transfection of HEK293T cells with PEI<\/a><\/p>\n<ul>\n<li>This is a cheap and effective protocol for transfecting HEK293T cells using PEI. \u00a0<em>Note: In Jan 2013 we posted an updated version (version 2) with clarifications requested by users.\u00a0<\/em>We have another variant\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/PEI-transfection.pdf\">here<\/a>.\u00a0We have adapted this protocol for 24 well plates. To see the adapted version click\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/01\/Protocol-for-24-Well-Plates.pdf\">Protocol for 24-Well Plates<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cloning \u00a0Site-directed mutagenesis Immunoprecipitation This protocol demonstrates how to immunoprecipitate a protein from a cell lysate. Co-immunoprecipitation Transfection of HEK293T cells with PEI This is a cheap and effective protocol for transfecting HEK293T cells using PEI. \u00a0Note: In Jan 2013 &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/general-molecular-biology\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2720","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2720","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2720"}],"version-history":[{"count":1,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2720\/revisions"}],"predecessor-version":[{"id":2721,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2720\/revisions\/2721"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2720"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}