{"id":2576,"date":"2018-08-12T14:37:28","date_gmt":"2018-08-12T18:37:28","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2576"},"modified":"2026-01-03T14:03:25","modified_gmt":"2026-01-03T19:03:25","slug":"detection-of-bacterial-products","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/detection-of-bacterial-products\/","title":{"rendered":"Detection of Bacterial Products"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/01\/LPS-Detection-Protocol_Updated.pdf\">Measuring LPS in plasma or serum<\/a><\/p>\n<ul>\n<li>This protocol is used for measuring levels of LPS in mouse or human plasma or serum.<\/li>\n<li>Citation:&nbsp;<a href=\"https:\/\/link.springer.com\/article\/10.1007%2Fs10753-014-0030-z\">Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007\/s10753-014-0030-z.<\/a><\/li>\n<\/ul>\n<p>M<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2021\/11\/Quantification-of-cell-free-DNA-and-mtDNA-June2017.docx\">easuring DNA in plasma or serum (16s rRNA, mitochondrial, nuclear)<\/a><\/p>\n<ul>\n<li>Citation:&nbsp;<a href=\"https:\/\/link.springer.com\/article\/10.1007%2Fs10753-014-0030-z\">Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone J, Loeb M, Bramson JL, Bowdish DM. Inflammation. 2015 Feb;38(1):272-7. doi: 10.1007\/s10753-014-0030-z.<\/a><\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/01\/MDP-Detection-Protocol_Updated.pdf\">Measuring muramyl dipeptide (MDP) in plasma or serum<\/a><\/p>\n<ul>\n<li>This protocol is used for measuring levels of MDP, a component of the bacterial cell wall in mouse or human plasma or serum.<\/li>\n<li>Citation: Age-<a href=\"http:\/\/www.cell.com\/cell-host-microbe\/fulltext\/S1931-3128(17)30112-9?_returnURL=http%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1931312817301129%3Fshowall%3Dtrue\">Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.N Thevaranjan, A Puchta, C Schulz, A Naidoo, JC Szamosi, \u2026Cell Host &amp; Microbe 21 (4), 455-466. e4<\/a><\/li>\n<\/ul>\n<p style=\"font-weight: inherit;\"><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/SEAP-Reporter-Assay.pdf\">Nf-kB-secreted alkaline phosphatase protocol using HEK293T cells<\/a><\/p>\n<ul style=\"font-weight: inherit;\">\n<li>This assay can be used to measure Nf-kB activation downstream of TLR or NLR expression.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/07\/NF-KB-Luciferase-Assay-Protocolv2.pdf\">NF-KB Luciferase Assay using HEK293T cells<\/a><\/p>\n<ul>\n<li>This protocol is used to measure activation of Nf-kb downstream of activation of TLRs or NLRs.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Measuring LPS in plasma or serum This protocol is used for measuring levels of LPS in mouse or human plasma or serum. Citation:&nbsp;Circulating muramyl dipeptide is negatively associated with interleukin-10 in the frail elderly.Verschoor CP, Naidoo A, Wallace JG, Johnstone &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/detection-of-bacterial-products\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2576","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2576","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2576"}],"version-history":[{"count":11,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2576\/revisions"}],"predecessor-version":[{"id":3774,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2576\/revisions\/3774"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2576"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}