{"id":2573,"date":"2018-12-24T07:32:14","date_gmt":"2018-12-24T12:32:14","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2573"},"modified":"2026-01-03T13:48:40","modified_gmt":"2026-01-03T18:48:40","slug":"streptococcus-pneumoniae","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/streptococcus-pneumoniae\/","title":{"rendered":"Microbiology &#038; Streptococcus pneumoniae"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/09\/Transformation-of-competent-cells.pdf\">Transformation of competent cells<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/03\/Culturing-Streptococcus-pneumoniae.pdf\">Growth and preparation of\u00a0<em>Streptococcus pneumoniae<\/em><\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/Making-Blood-Plates-Protocol-.pdf\">Making sheep\u2019s blood agar plates for culture of\u00a0<em>Streptococcus pneumoniae<\/em><\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/02\/Streptococcus-pneumoniae-colonization1.pdf\">Intranasal colonization of\u00a0<em>Streptococcus pneumoniae<\/em>\u00a0(and measurement of gene expression and leukocyte recruitment to the nasopharnyx)<\/a><\/p>\n<ul>\n<li>This is a detailed protocol for preparing\u00a0<em>Streptococcus pneumoniae\u00a0<\/em>and intranasal colonization of mice. Puchta A, Verschoor CP, Thurn T, Bowdish DM.\u00a0<a href=\"http:\/\/www.jove.com\/video\/50490\/characterization-inflammatory-responses-during-intranasal\">Characterization of inflammatory responses during intranasal colonization with\u00a0<\/a><a href=\"http:\/\/www.jove.com\/video\/50490\/characterization-inflammatory-responses-during-intranasal\"><em>Streptococcus pneumoniae.<\/em><\/a><em>\u00a0<\/em>J Vis Exp. 2014 Jan 17;(83):e50490. doi: 10.3791\/50490. For access to an accompanying video, please contact Dr. Bowdish (bowdish@mcmaster.ca)<\/li>\n<li>We have a protocol specific to TIGR4\/P1542\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/streptococcus-pneumoniae\/_wp_link_placeholder\">here<\/a>.<\/li>\n<li>Also used in:\u00a0<a href=\"http:\/\/iai.asm.org\/cgi\/pmidlookup?view=long&amp;pmid=26787714\">Thevaranjan N, et al. Streptococcus pneumoniae Colonization Disrupts the Microbial Community within the Upper Respiratory Tract of Aging Mice.Infect Immun. 2016 Mar 24;84(4):906-16. doi: 10.1128\/IAI.01275-15.\u00a0<\/a><\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2019\/09\/Pneumolysin-expression_purification_hemolysis.pdf\">Pneumolysin purification from an expression plasmid<\/a>.<\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/07\/CFSE-labeling-of-S-pneumoniae.pdf\">CFSE labelling of<em>\u00a0S pneumoniae<\/em><\/a><\/p>\n<ul>\n<li>This protocol uses CFSE to label\u00a0<em>S. pneumoniae<\/em>\u00a0for flow cytometry or microscopy experiments.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Pneumococcal-Cell-Wall-Purification.pdf\">Pneumococcal Cell Wall Purification<\/a><\/p>\n<ul>\n<li>This protocol describes how to purify the pneumococcal cell wall. The purified preparation will not contain proteins but will contain the peptidoglycan layer and teichoic acids.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Mannose-Detection-Protocol.pdf\">Phenol-sulfuric acid Mannose detection assay<\/a>.<\/p>\n<ul>\n<li>This assay is sensitive to mannose from 1 \u2013 100 ?g but will also detect\u00a0other carbohydrates. We use this assay to\u00a0quantify of complex carbohydrates in the bacterial cell wall<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Transformation of competent cells Growth and preparation of\u00a0Streptococcus pneumoniae Making sheep\u2019s blood agar plates for culture of\u00a0Streptococcus pneumoniae Intranasal colonization of\u00a0Streptococcus pneumoniae\u00a0(and measurement of gene expression and leukocyte recruitment to the nasopharnyx) This is a detailed protocol for preparing\u00a0Streptococcus pneumoniae\u00a0and &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/streptococcus-pneumoniae\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3584,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2573","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2573","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2573"}],"version-history":[{"count":7,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2573\/revisions"}],"predecessor-version":[{"id":3762,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2573\/revisions\/3762"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3584"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2573"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}