{"id":2569,"date":"2018-08-11T21:00:51","date_gmt":"2018-08-12T01:00:51","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2569"},"modified":"2026-01-03T14:02:54","modified_gmt":"2026-01-03T19:02:54","slug":"elisas","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/elisas\/","title":{"rendered":"Cytokine measurements (ELISA &#038; others)"},"content":{"rendered":"<h1>General<\/h1>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\"><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/cytokine-ELISA.pdf\">Cytokine ELISA&nbsp;<\/a><\/p>\n<ul>\n<li>This is a general protocol for performing ELISAs.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\"><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/02\/ELISA-titration.pdf\">How to titrate antibodies for DIY ELISAs<\/a><\/p>\n<ul>\n<li>It&#8217;s often cheaper to buy the components of an ELISA (i.e. standard, capture &amp; detecting antibodies) to make your own ELISA than to buy commercial ones. Here is an easy to follow protocol on how to systematically determine if you are using the right concentrations of antibodies.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\"><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2021\/11\/2021-07-22-EllaProtocolBowdish.docx\">Using the Ella SimplePlex System to measure cytokines<\/a><\/p>\n<h1>Specific ELISAs<\/h1>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\">Human&nbsp;<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/08\/Human-CRP-ELISA-Updated.pdf\">C-reactive protein (CRP)<\/a><\/p>\n<ul>\n<li>Citation: <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S05315\">Verschoor CP, et al.DNA methylation patterns are related to co-morbidity status and circulating C-reactive protein levels in the nursing home elderly.Exp Gerontol. 2018 May;105:47-52. doi: 10.1016\/j.exger.2017.10.010<\/a><\/li>\n<\/ul>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>General Cytokine ELISA&nbsp; This is a general protocol for performing ELISAs. How to titrate antibodies for DIY ELISAs It&#8217;s often cheaper to buy the components of an ELISA (i.e. standard, capture &amp; detecting antibodies) to make your own ELISA than &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/elisas\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2569","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2569","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2569"}],"version-history":[{"count":4,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2569\/revisions"}],"predecessor-version":[{"id":3773,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2569\/revisions\/3773"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2569"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}