{"id":2564,"date":"2018-12-24T07:24:16","date_gmt":"2018-12-24T12:24:16","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2564"},"modified":"2026-01-03T14:02:08","modified_gmt":"2026-01-03T19:02:08","slug":"blood-processing-leukocyte-isolation","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/blood-processing-leukocyte-isolation\/","title":{"rendered":"Blood Processing &#038; Leukocyte Isolation"},"content":{"rendered":"<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2020\/12\/2020-12-Isolation_and_cryopreservation_of_human_serum_protocol-v2.pdf\">Cryopreservation of serum<\/a><\/li>\n<li>I<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/03\/PBMC-Banking-Protocol-Using-Leucosep-Tubes-Formatted.pdf\">solation of&nbsp;peripheral blood mononuclear cells (PBMC) using Leucosep tubes and cryopreservation<\/a>.<\/li>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/03\/PBMC-and-Granulocyte-Banking-Protocol-Formatted.pdf\">Isolation of peripheral blood mononuclear cells (PBMC) and granulocytes using a Ficoll gradient and subsequent cryopreservation<\/a>.\n<ul>\n<li>Note that the granulocytes are not viable after this technique but they are suitable for analysis of RNA\/DNA<\/li>\n<\/ul>\n<\/li>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2021\/12\/Phlebotomy.pdf\">Phlebotomy<\/a>&nbsp;<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Cryopreservation of serum Isolation of&nbsp;peripheral blood mononuclear cells (PBMC) using Leucosep tubes and cryopreservation. Isolation of peripheral blood mononuclear cells (PBMC) and granulocytes using a Ficoll gradient and subsequent cryopreservation. Note that the granulocytes are not viable after this technique &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/blood-processing-leukocyte-isolation\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3584,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2564","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2564"}],"version-history":[{"count":10,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2564\/revisions"}],"predecessor-version":[{"id":3772,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2564\/revisions\/3772"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3584"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}