{"id":2545,"date":"2018-08-10T21:01:19","date_gmt":"2018-08-11T01:01:19","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2545"},"modified":"2025-01-05T09:11:30","modified_gmt":"2025-01-05T14:11:30","slug":"antibody-production-purification","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/antibody-production-purification\/","title":{"rendered":"Antibody Production &#038; Purification"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Protein-A-Sepharose-purification-of-Antibodies.pdf\">Protein A Sepharose purification of Antibodies<\/a><\/p>\n<ul>\n<li>This protocol is useful for purifying antibodies from hybridoma cultures and serum.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/09\/Generation-of-Rabbit-Polyclonal-Antibodies-2016-KN-Update.pdf\">Immunization protocol for rabbits<\/a><\/p>\n<ul>\n<li>This protocol is used for generating polyclonal antibodies to peptide ligands in rabbits.<\/li>\n<li>Citation:\u00a0<a href=\"https:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1038\/icb.2016.20\">Novakowski KE, et al.A naturally occurring transcript variant of MARCO reveals the SRCR domain is critical for function. Immunol Cell Biol. 2016 Aug;94(7):646-55.<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Protein A Sepharose purification of Antibodies This protocol is useful for purifying antibodies from hybridoma cultures and serum. Immunization protocol for rabbits This protocol is used for generating polyclonal antibodies to peptide ligands in rabbits. Citation:\u00a0Novakowski KE, et al.A naturally &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/antibody-production-purification\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3584,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2545","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2545","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2545"}],"version-history":[{"count":4,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2545\/revisions"}],"predecessor-version":[{"id":3608,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2545\/revisions\/3608"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3584"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}