{"id":2543,"date":"2018-12-24T07:19:07","date_gmt":"2018-12-24T12:19:07","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2543"},"modified":"2018-12-29T09:56:39","modified_gmt":"2018-12-29T14:56:39","slug":"bacterial-killing-assays","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/bacterial-killing-assays\/","title":{"rendered":"Bacterial Killing Assays"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/macrophage-killing-assay-protocol\/\">Macrophage Killing Assay<\/a><\/p>\n<ul>\n<li>How do you measure how well macrophages kill bacteria (or whether your treatment alters killing)? This is a standard kill curve protocol and a workhorse of the Bowdish lab. Specifically written to measure\u00a0<em>S. pneumoniae<\/em>\u00a0killing, can be adapted for any bacteria and any type of macrophage (e.g. human, murine, alveolar, bone marrow,etc).<\/li>\n<li>Citation: <a href=\"https:\/\/www.cell.com\/cell-host-microbe\/fulltext\/S1931-3128(17)30112-9?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1931312817301129%3Fshowall%3Dtrue\">Thevaranjan N, et al. Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.Cell Host Microbe. 2017 Apr 12;21(4):455-466.e4. doi: 10.1016\/j.chom.2017.03.002.\u00a0<\/a>PMID: 28407483<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Macrophage Killing Assay How do you measure how well macrophages kill bacteria (or whether your treatment alters killing)? This is a standard kill curve protocol and a workhorse of the Bowdish lab. Specifically written to measure\u00a0S. pneumoniae\u00a0killing, can be adapted &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/bacterial-killing-assays\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2543","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2543","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2543"}],"version-history":[{"count":2,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2543\/revisions"}],"predecessor-version":[{"id":2647,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2543\/revisions\/2647"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2543"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}