{"id":2533,"date":"2018-08-10T20:48:08","date_gmt":"2018-08-11T00:48:08","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2533"},"modified":"2026-01-03T13:44:51","modified_gmt":"2026-01-03T18:44:51","slug":"macrophage-isolation-culture","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/macrophage-isolation-culture\/","title":{"rendered":"Macrophage Isolation &#038; Culture (primary cells)"},"content":{"rendered":"<h2>For macrophage cell lines see &#8216;Tissue Culture&#8217; section<\/h2>\n<h2>Murine<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2019\/08\/Culturing-Freezing-Defrosting-Bone-Marrow-Derived-Macrophages.pdf\">Culture, freezing and defrosting murine bone marrow macrophages<\/a><\/li>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/03\/Harvesting-BG-pM%C3%B8s.pdf\">Elicitation and collection of peritoneal macrophages and neutrophils using Biogel<\/a><\/li>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2025\/11\/protocol-femur-BM-extraction-2024-11-14.pdf\">Harvesting murine bone marrow\u00a0from femurs<\/a><\/li>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2020\/01\/Isolation-of-murine-spinal-bone-marrow.pdf\">Harvesting murine bone marrow from spines<\/a>\n<ul>\n<li>We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur &amp; tibias. For an accompanying video, please contact Dr. Bowdish (<a href=\"mailto:bowdish@mcmaster.ca\">bowdish@mcmaster.ca<\/a>)<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<h2>Human<\/h2>\n<ul>\n<li><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Human-Macrophage-Culture-2.pdf\">Human monocyte derived macrophage culture<\/a>\n<ul>\n<li>Citation:\u00a0<a href=\"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0198-8859(14)00469-8\">Verschoor CP, et al. Anti-pneumococcal deficits of monocyte-derived macrophages from the advanced-age, frail elderly and related impairments in PI3K-AKT signaling.Hum Immunol. 2014 Dec;75(1\\2):1192-6<\/a>.<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>For macrophage cell lines see &#8216;Tissue Culture&#8217; section Murine Culture, freezing and defrosting murine bone marrow macrophages Elicitation and collection of peritoneal macrophages and neutrophils using Biogel Harvesting murine bone marrow\u00a0from femurs Harvesting murine bone marrow from spines We have &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/macrophage-isolation-culture\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2533","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2533","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2533"}],"version-history":[{"count":15,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2533\/revisions"}],"predecessor-version":[{"id":3759,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2533\/revisions\/3759"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2533"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}