{"id":2311,"date":"2018-12-24T07:29:46","date_gmt":"2018-12-24T12:29:46","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2311"},"modified":"2026-01-03T13:50:04","modified_gmt":"2026-01-03T18:50:04","slug":"phagocytosis-endocytosis","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols\/phagocytosis-endocytosis\/","title":{"rendered":"Phagocytosis &#038; Endocytosis"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/08\/Measuring-uptake-using-protein-coated-beads.pdf\">Measuring endocytosis\/phagocytosis of polystyrene or protein coated beads<\/a><\/p>\n<ul>\n<li>Citation:\u00a0<a href=\"https:\/\/academic.oup.com\/mbe\/article-abstract\/35\/2\/440\/4642931?redirectedFrom=fulltext\">Novakowski KE, Yap NVL, Yin C, Sakamoto K, Heit B, Golding GB, Bowdish DME. Human-Specific Mutations and Positively Selected Sites in MARCO Confer Functional Changes. Mol Biol Evol. 2018 Feb 1;35(2):440-450. doi:10.1093\/molbev\/msx298.<\/a>PMID: 29165618<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/03\/Macrophage-Killing-Assay.pdf\">Macrophage Killing Assay<\/a><\/p>\n<ul>\n<li>How do you measure how well macrophages kill bacteria (or whether your treatment alters killing)? This is a standard kill curve protocol and a workhorse of the Bowdish lab. Specifically written to measure\u00a0<em> pneumoniae<\/em>killing, can be adapted for any bacteria and any type of macrophage (e.g. human, murine, alveolar, bone marrow,etc).<\/li>\n<li>Citation:\u00a0<a href=\"https:\/\/www.cell.com\/cell-host-microbe\/fulltext\/S1931-3128(17)30112-9?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1931312817301129%3Fshowall%3Dtrue\">Thevaranjan N, et al. Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability, Systemic Inflammation, and Macrophage Dysfunction.Cell Host Microbe. 2017 Apr 12;21(4):455-466.e4. doi: 10.1016\/j.chom.2017.03.002.\u00a0<\/a>PMID: 28407483<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/REAL-TIME-SPECTROFLUOROMETRIC-PHAGOLYSOSOMAL-FUSION-ASSAY-.pdf\">Phagolysosomal fusion assay<\/a><\/p>\n<ul>\n<li>Adapted from:<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19347317\">\u00a0Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities. Yates RM, Hermetter A, Russell DG. Methods Mol Biol. 2009;531:157-71. doi: 10.1007\/978-1-59745-396-7_11. PMID: 19347317<\/a><\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/Intraphagosomal-Assay-Particle-Preparation-Protocol-.pdf\">Preparation of\u00a0 mannosylated beads for phagolysosomal fusion assays<\/a><\/p>\n<ul>\n<li style=\"list-style-type: none;\">\n<ul>\n<li>Adapted from:<a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/19347317\">\u00a0Recording phagosome maturation through the real-time, spectrofluorometric measurement of hydrolytic activities. Yates RM, Hermetter A, Russell DG. Methods Mol Biol. 2009;531:157-71. doi: 10.1007\/978-1-59745-396-7_11. PMID: 19347317<\/a><\/li>\n<\/ul>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Measuring endocytosis\/phagocytosis of polystyrene or protein coated beads Citation:\u00a0Novakowski KE, Yap NVL, Yin C, Sakamoto K, Heit B, Golding GB, Bowdish DME. Human-Specific Mutations and Positively Selected Sites in MARCO Confer Functional Changes. Mol Biol Evol. 2018 Feb 1;35(2):440-450. doi:10.1093\/molbev\/msx298.PMID: &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols\/phagocytosis-endocytosis\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":3736,"parent":2428,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2311","page","type-page","status-publish","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2311","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2311"}],"version-history":[{"count":8,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2311\/revisions"}],"predecessor-version":[{"id":3763,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2311\/revisions\/3763"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2428"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media\/3736"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2311"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}