{"id":2280,"date":"2018-03-29T16:16:06","date_gmt":"2018-03-29T20:16:06","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=2280"},"modified":"2018-03-29T16:16:06","modified_gmt":"2018-03-29T20:16:06","slug":"isolation-and-cryopreservation-of-leukocytes","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/isolation-and-cryopreservation-of-leukocytes\/","title":{"rendered":"Isolation and cryopreservation of leukocytes"},"content":{"rendered":"<p>I<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/03\/PBMC-Banking-Protocol-Using-Leucosep-Tubes-Formatted.pdf\">solation of\u00a0peripheral blood mononuclear cells (PBMC) using Leucosep tubes and cryopreservation<\/a>.<\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/03\/PBMC-and-Granulocyte-Banking-Protocol-Formatted.pdf\">Isolation of\u00a0peripheral blood mononuclear cells (PBMC) and granulocytes using a Ficoll gradient and subsequent cryopreservation<\/a>.<\/p>\n<ul>\n<li>Note that the granulocytes are not viable after this technique but they are suitable for analysis of RNA\/DNA<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Isolation of\u00a0peripheral blood mononuclear cells (PBMC) using Leucosep tubes and cryopreservation. Isolation of\u00a0peripheral blood mononuclear cells (PBMC) and granulocytes using a Ficoll gradient and subsequent cryopreservation. Note that the granulocytes are not viable after this technique but they are suitable &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/isolation-and-cryopreservation-of-leukocytes\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-2280","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2280","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=2280"}],"version-history":[{"count":1,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2280\/revisions"}],"predecessor-version":[{"id":2283,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/2280\/revisions\/2283"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=2280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}