{"id":1606,"date":"2014-11-16T22:11:30","date_gmt":"2014-11-17T03:11:30","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=1606"},"modified":"2018-12-29T10:12:33","modified_gmt":"2018-12-29T15:12:33","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/flow-cytometry\/","title":{"rendered":"Flow cytometry (old)"},"content":{"rendered":"<h2>General<\/h2>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2015\/07\/Verschoor-Flow-cytometry-gating-tools.pdf\">An introduction to automated flow cytometry gating tools and their implementation<\/a><\/p>\n<ul>\n<li>This review on automated gating tool for use in multi-parameter flow cytometry is published in\u00a0<i>Front. Immunol.<\/i> 6:380. doi: 10.3389\/fimmu.2015.00380<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Flow-cytometry-staining.pdf\">General Flow Cytometry protoco<\/a>l<\/p>\n<h2>Bacterial flow cytometry<\/h2>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/06\/Flow-Cytometry-Binding-Assay.pdf\">Measurement of binding to intact bacteria<\/a><\/p>\n<ul>\n<li>This protocol is used for &#8220;bacterial flow cytometry&#8221; (running bacteria through the flow cytometer rather than eukaroytic cells). We measure protein binding to the bacteria but it could easily be adapted to antibodies.<\/li>\n<\/ul>\n<h2><\/h2>\n<h2>Humans<\/h2>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/04\/Human-Monocyte-Stain-Protocol.pdf\">\u00a0Human monocyte staining protocol<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/04\/Human-Lymphocyte-Stain-Protocol.pdf\">\u00a0<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/05\/Lymphocyte-Stain-Protocol.pdf\">Human lymphocyte staining protocol<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/05\/Reg-T-cell-Bowdish-Protocol.pdf\">Human Treg (regulatory T cell) staining<\/a><\/p>\n<h2>Mice<\/h2>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/02\/Monocyte-Surface-Stain-Protocol-2018.pdf\">Flow cytometry protocol for staining Ly6Chigh and Ly6Clow monocytes<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>General An introduction to automated flow cytometry gating tools and their implementation This review on automated gating tool for use in multi-parameter flow cytometry is published in\u00a0Front. Immunol. 6:380. doi: 10.3389\/fimmu.2015.00380 General Flow Cytometry protocol Bacterial flow cytometry Measurement of &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/flow-cytometry\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1606","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1606","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=1606"}],"version-history":[{"count":9,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1606\/revisions"}],"predecessor-version":[{"id":2713,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1606\/revisions\/2713"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=1606"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}