{"id":1600,"date":"2014-11-16T22:08:17","date_gmt":"2014-11-17T03:08:17","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=1600"},"modified":"2017-08-17T16:10:49","modified_gmt":"2017-08-17T20:10:49","slug":"molecular-biology","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/molecular-biology\/","title":{"rendered":"Molecular Biology"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Molecular-Cloning.pdf\">Cloning<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Immunoprecipitation.pdf\"><br \/>\n<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/04\/qPCR-Bible.pdf\">The PCR resource manual.<\/a><\/p>\n<ul>\n<li>The how-to guide:qPCR from primer design to testing those primers in preparation for quantitative PCR<\/li>\n<\/ul>\n<p><em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<\/em><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2017\/08\/qPCR-Primer-Design.pdf\">Designing primers for qPCR<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2012\/10\/Immunoprecipitation.pdf\">Immunoprecipitation<\/a><\/p>\n<ul>\n<li>This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Co-Immunoprecipitation.pdf\">Co-immunoprecipitation<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/06\/Western-Blottingv2.pdf\">Western immunoblotting<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Coomassie-Blue-Stain-Protocol.pdf\">Coommassie blue staining of protein gels<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/04\/Site-Directed-Mutagenesis-Protocol.pdf\">Site-directed mutagenesis<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/01\/PEI_Transfection_Bowdish_v2.pdf\">PEI_Transfection_Bowdish_v2<\/a><\/p>\n<ul>\n<li>This is a cheap and effective protocol for transfecting HEK293T cells using PEI. \u00a0<em>Note: In Jan 2013 we posted an updated version (version 2) with clarifications requested by users.\u00a0<\/em>We have another variant\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/PEI-transfection.pdf\">here<\/a>.\u00a0We have adapted this protocol for 24 well plates. To see the adapted version click\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/01\/Protocol-for-24-Well-Plates.pdf\">Protocol for 24-Well Plates<\/a><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Cloning The PCR resource manual. The how-to guide:qPCR from primer design to testing those primers in preparation for quantitative PCR \u00a0Designing primers for qPCR Immunoprecipitation This protocol demonstrates how to immunoprecipitate a protein from a cell lysate. Co-immunoprecipitation Western immunoblotting &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/molecular-biology\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1600","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1600","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=1600"}],"version-history":[{"count":6,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1600\/revisions"}],"predecessor-version":[{"id":2188,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1600\/revisions\/2188"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=1600"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}