{"id":1598,"date":"2014-11-16T22:07:45","date_gmt":"2014-11-17T03:07:45","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=1598"},"modified":"2018-12-24T07:33:43","modified_gmt":"2018-12-24T12:33:43","slug":"cell-culture","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/cell-culture\/","title":{"rendered":"Cell Culture\/Tissue Culture"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Cell-Count.pdf\">Counting cells with a hemocytometer and measuring viability with Trypan Blue.\u00a0<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/THP-1-propagation-culture.pdf\">Propagation &amp; culture of THP-1 cells<\/a><\/p>\n<ul>\n<li>\u00a0Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/05\/Culturing-RAW264.7-Cells.pdf\">Culturing RAW 264.7 Cells<\/a><\/p>\n<ul>\n<li>The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. This is an update of the <a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/02\/Culturing-RAW264.7-Cells.pdf\">original<\/a> in\u00a0we describe culture &amp; propogation of the cells and include information on seeding density for studies of cytokine production.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Culture-of-HEK-cells.pdf\">Culturing HEK293\/HEK293T cells<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2016\/04\/Culturing-Maintaining-and-Seeding-Detroit-562-Cells-1.pdf\">Culturing, Maintaining and Seeding the nasopharyngeal cell line Detroit 562<\/a><\/p>\n<ul>\n<li>This\u00a0human derived nasopharyngeal carcinoma cell line maintains many of the properties of nasopharyngeal epithelial cells.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2018\/12\/L929-CONDITIONED-MEDIUM-LCM-Protocol-.pdf\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" alt=\"\" width=\"16\" height=\"16\" \/>Growing L929 cells and collecting conditioned media for macrophage culture<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0Counting cells with a hemocytometer and measuring viability with Trypan Blue.\u00a0 Propagation &amp; culture of THP-1 cells \u00a0Tips and tricks for growing this popular, but finicky, human monocyte-like cell line. \u00a0Culturing RAW 264.7 Cells The murine RAW 264.7 cell line &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/cell-culture\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1598","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1598","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=1598"}],"version-history":[{"count":7,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1598\/revisions"}],"predecessor-version":[{"id":2654,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1598\/revisions\/2654"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=1598"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}