{"id":1529,"date":"2014-10-21T14:32:31","date_gmt":"2014-10-21T18:32:31","guid":{"rendered":"https:\/\/www.bowdish.ca\/lab\/?page_id=1529"},"modified":"2014-11-26T16:55:59","modified_gmt":"2014-11-26T21:55:59","slug":"macrophage-isolation-culture-2","status":"publish","type":"page","link":"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-isolation-culture-2\/","title":{"rendered":"Macrophage Isolation &#038; Culture"},"content":{"rendered":"<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Harvesting_murine_bone-marrow.pdf\">Harvesting murine bone marrow<\/a>\u00a0from femurs and tibia<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"16\" height=\"16\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/11\/Human-Macrophage-Culture-2.pdf\">Human monocyte derived macrophage culture<\/a><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"16\" height=\"16\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/10\/Spinal-BM-Isolation-.pdf\">Harvesting murine\u00a0bone marrow from spines<\/a><\/p>\n<ul>\n<li>We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur &amp; tibias.<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Isolation_and_culture_of_murine_bone_marrow_macrophages.pdf\">Isolation &amp; culture of murine bone marrow macrophages<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2014\/03\/Harvesting-BG-pM\u00f8s.pdf\">Elicitation and collection of peritoneal macrophages and neutrophils using Biogel<\/a><\/p>\n<p><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\"><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"20\" height=\"20\" \/><\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/THP-1-propagation-culture.pdf\">Propagation &amp; culture of THP-1 cells<\/a><\/p>\n<ul>\n<li>\u00a0Tips and tricks for growing this popular, but finicky, human monocyte-like cell line.<\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"16\" height=\"16\" \/>\u00a0<a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2013\/02\/Culturing-RAW264.7-Cells.pdf\">Culturing RAW264.7 Cells<\/a><\/p>\n<ul>\n<li>The murine RAW 264.7 cell line is considered one of the best macrophage cell lines. Here we describe culture &amp; propogation of the cells.<\/li>\n<\/ul>\n<div><img loading=\"lazy\" decoding=\"async\" title=\"Macrophage-bullet\" alt=\"\" src=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Macrophage-bullet.gif\" width=\"16\" height=\"16\" \/><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">Calcein staining of<\/a><a href=\"https:\/\/www.bowdish.ca\/lab\/wp-content\/uploads\/2011\/07\/Calcein_staining_of_macrophages.pdf\">\u00a0macrophages<\/a><\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Harvesting murine bone marrow\u00a0from femurs and tibia Human monocyte derived macrophage culture Harvesting murine\u00a0bone marrow from spines We have found that you get as much as 5x more bone marrow when collecting from the spines, as opposed to standard femur &hellip; <a href=\"https:\/\/www.bowdish.ca\/lab\/protocols-old\/macrophage-isolation-culture-2\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":436,"menu_order":0,"comment_status":"open","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-1529","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1529","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/comments?post=1529"}],"version-history":[{"count":4,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1529\/revisions"}],"predecessor-version":[{"id":1578,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/1529\/revisions\/1578"}],"up":[{"embeddable":true,"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/pages\/436"}],"wp:attachment":[{"href":"https:\/\/www.bowdish.ca\/lab\/wp-json\/wp\/v2\/media?parent=1529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}