Molecular Biology

Cloning

Immunoprecipitation

  • This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.

Co-immunoprecipitation

Western immunoblotting

 Coommassie blue staining of protein gels

 Site-directed mutagenesis

PEI_Transfection_Bowdish_v2

  • This is a cheap and effective protocol for transfecting HEK293T cells using PEI.  Note: In Jan 2013 we posted an updated version (version 2) with clarifications requested by users. We have another variant here. We have adapted this protocol for 24 well plates. To see the adapted version click Protocol for 24-Well Plates

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