Molecular Biology


The PCR resource manual.

  • The how-to guide:qPCR from primer design to testing those primers in preparation for quantitative PCR

 Designing primers for qPCR


  • This protocol demonstrates how to immunoprecipitate a protein from a cell lysate.


Western immunoblotting

 Coommassie blue staining of protein gels

 Site-directed mutagenesis


  • This is a cheap and effective protocol for transfecting HEK293T cells using PEI.  Note: In Jan 2013 we posted an updated version (version 2) with clarifications requested by users. We have another variant here. We have adapted this protocol for 24 well plates. To see the adapted version click Protocol for 24-Well Plates

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